IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Allogeneic and syngeneic cells as antigen source and their efficiency in DC-based anti-melanoma vaccination
Autor/es:
MAC KEON, SOLEDAD; MORDOH, JOSÉ; VENTIVEGNA, SOFÍA; ROSA WAINSTOK; LEVY, ESTRELLA MARIEL
Lugar:
Buenos Aires
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Institución organizadora:
SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; SOCIEDAD ARGENTINA DE INVESTIGACIÓN BIOQUÍMICA Y BIOLOGÍA MOLECULAR; SOCIEDAD ARGENTINA DE INMUNOLOGÍA;
Resumen:
A major obstacle to obtaining relevant results in cancer vaccinationhas been the identification of immunogenic antigens. In particular,dendritic cell (DC)-based cancer immunotherapy can be achievedby loading DCs with syngeneic or allogeneic cells, but the consequencesbrought upon anti-tumor protection are not clear. Whenusing syngeneic tumor cells, tumor self-antigens, including cancertestis antigens (CTA), other tumor-associated antigens (TAA) andneoantigens generated through mutations during tumor progression,are provided. On the other hand, allogeneic tumor cells couldonly supply shared CTA and other TAA. To assess the advantagesof each system, we have analyzed in a murine model the effect onanti-melanoma protection of loading DCs with irradiated syngeneicB16-F1 melanoma, allogeneic Cloudman melanoma, or allogeneic4T1 mammary carcinoma cells (DC-ApoNec vaccines). The cellswere fully characterized by whole Exome Sequencing and RNAseq.Using Spinning Disk Confocal Microscopy we observed that irradiatedtumor cell components were efficiently internalized by DCs, andtransported to MHC-class II-positive tubulovesicular compartments.Loading DCs with allogeneic cells induced significantly higher DCmaturation than syngeneic cells, measured by Flow Cytometry asCD86 and I-Ab surface expression. DC-ApoNec vaccines were administeredto C57Bl6 mice, followed by B16-F1 tumoral challenge.Allogeneic melanoma cells induced effective anti-melanoma protection(p