IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IN VITRO SECRETOMIC ANALYSIS OF PROSTATE CANCER CELLS AND BONE PROGENITOR CELLS GROWING IN CO-CULTURE SYSTEMS.
Autor/es:
GUERON, GERALDINE; COTIGNOLA, JAVIER; ANSELMINO, NICOLAS; VALACCO, PIA; PAEZ, ALEJANDRA ; VAZQUEZ, ELBA; LAGE VICKERS, SOFIA; GUERON, GERALDINE; COTIGNOLA, JAVIER; ANSELMINO, NICOLAS; VALACCO, PIA; PAEZ, ALEJANDRA ; VAZQUEZ, ELBA; LAGE VICKERS, SOFIA
Reunión:
Congreso; Sociedad Argentina de Investigación clínica; 2017
Institución organizadora:
SAIC
Resumen:
Currently our view of cancer has evolved to include, in addition to the transformed cells that have deregulated homeostatic mechanisms, a wide spectrum of cells of the tumor microenvironment. The dialogue established between the tumor cells and its microenvironment, is an essential determinant of the characteristics of the tumor progression. The dissemination profile of Prostate Cancer (PCa) shows tendency to develop in the bone, where tumoral cells interact with the microenvironment disrupting the bone tissue balance. This work aimed at analyzing, which soluble factors could be potential mediators of the chemical cross-talk between PCa cells and bone progenitor cells. For this purpose, we used co-culture transwell systems of PC3 (human PCa cells) with the pre-osteoclastic Raw264.7 or pre-osteoblastic MC3T3 cell lines (murine cells), where cells shared the culture media (CMs) without physical contact for 24h.We employed mass spectrometry -nanoLC-MS/MS(Orbitrap)- for the secretome analysis of the CMs. The obtained spectra were analyzed with the Proteome Discover Software and compared with both, human and murine protein databases. Results highlight a differential profile of proteins released to the CM when tumor cells are grown in co-culture compared to controls. These results could potentially explain the altered mRNA expression levels assessed by RTqPCR in PCa cells growing in-culture, displaying significant up-regulation for HO-1 ANXA2, ANXA2R,OPG and PTHrP (p