Fibremicroparticles obtained from japanese plum (prunussalicina) have antioxidant activity in human cells

BASANTA M.F.; GARCIA G.A.; ERLEJMAN A.G.; CAMISAY M.F.; ROJAS A.M.; DE LEO S.A.

CABA

Jornada; Jornadas Interdisciplinarias de Química Biológica; 2017

Departamento de Química Biológica FCEN UBA

Japanese plums are largely grown for fresh consumption. Plums discarded bythe food industry can be used as an important source of bioactive compounds such as antioxidant polyphenols as well as dietary fibres like pectins. Polyphenols are habitually co-extracted with fibres. Certain polyphenols can exert cytoprotective actions in the gastrointestinal tract interfering with the oxidative stress implicated in inflammatory disorders. Fibremicroparticles (MPCs) obtained through ethanolic extraction from plums retained polyphenolic compounds, mainly pentamericproanthocyanidins (170?200mg/100g MPCs) and smaller amounts of anthocyanins and flavonoids. The aim of this work was to biologically evaluate in vitro the polyphenols extracted from plum MPCs in relation to their capability to protect colorectal epithelium (Caco-2) human cells and embryonic kidney (HEK293) cells against the oxidative stress induced by tert-butylhydroperoxide (tBOOH), as well as in their cytotoxicity. Cellular oxidation induced by t-BOOH was evaluated through the dichlorofluorescein (DCF) oxidation. When Caco-2 cells were co-incubated with 0.5 to 10μg/mL, the tBOOH induced oxidative stress was significantly reduced (p<0.001), showing a dependence on the concentration, reaching 100% of protection with 10μg/mL extract. A similar result was obtained in HEK293 cells where polyphenol extract showed significant protection (100%) against oxidation with 3 μg/mL of extract (p<0.05). Cell viability was measured in cells using the MTT assay (dimethyl tetrazole). Both Caco-2 and HEK293cells treated for 24 h with the extract (3 to 100 μg/ml concentration) presented a CC50>100 μg/mL. Therefore, polyphenol extracts showed a protective effect against oxidative stress coupled with low cytotoxicity. Plum MPCs obtained with co-extracted phenolics can be useful as a functional antioxidant dietary fibre, interfering with the oxidative stress implicated in inflammatory bowell disorders.