IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LONG-LIVED BINDING OF SOX2 TO DNA PREDICTS CELL FATE IN THE FOUR-CELL MOUSE EMBRYO
Autor/es:
NICOLAS PLACHTA; LUCIANA BRUNO; KAUR GRUPPERT; ALVAREZ, YANINA; STEPHANIE BISSIERE; ZIQING ZHAO; MELANIE D. WHITE; VALERIA LEVI; ESTEBAN MOCSKO; JUAN F. ANGIOLINI
Reunión:
Workshop; 17-20 APR 2018: EMBO Workshop on Nuclear Mechano Genomics; 2018
Resumen:
Transcription factor (TF) binding to DNA is fundamental for gene regulation. However, it remains unknown how the dynamics of TF-DNA interactions change during cell-fate determination in vivo. Here, we use photo-activatable FCS to quantify TF-DNA binding in single cells of developing mouse embryos. In blastocysts, the TFs Oct4 and Sox2, which control pluripotency, bind DNA more stably in pluripotent than in extraembryonic cells. By contrast, in the four-cell embryo, Sox2 engages in more long-lived interactions than does Oct4. Sox2 long-lived binding varies between blastomeres and is regulated by H3R26 methylation. Live-cell tracking demonstrates that those blastomeres with more long-lived binding contribute more pluripotent progeny, and reducing H3R26 methylation decreases long-lived binding, Sox2 target expression, and pluripotent cell numbers. Therefore, Sox2-DNA binding predicts mammalian cell fate as early as the four-cell stage. More generally, we reveal the dynamic repartitioning of TFs between DNA sites driven by physiological epigenetic changes.