IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The presence of homocysteine and adenosine exacerbated the tnf-α cytotoxicity in human erythroleukemia cells
Autor/es:
NESSE, A; SHIAPPACASSE, A; VITTORI, D; MALTANERI, R
Lugar:
Mar del Plata
Reunión:
Congreso; LXII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2017
Resumen:
Some factors are known to influence cell survival regulation, including TNF-α and cellular hypomethylation, which can be induced by high levels of homocysteine and adenosine. S-adenosylhomocysteine (SHA) production is accelerated by the reversible enzyme SAH hydrolase during the methionine metabolism. Both, hyperhomocysteinemia (hHcy) and elevated SAH has been described in end stage renal disease (ESRD) and associated with several cellular abnormalities in these patients. Other negative prognosis factors in ESRD are inflammatory conditions leading to anemia. The aim of this study was to investigate if hHcy and the accumulation of adenosine could affect the survival of human erythroleukemia K562 cells in an inflammatory environment and whether the growth factor erythropoietin (Epo) could reverse this effect.Cells were incubated (2 h) with Hcy (H 500 µM) and adenosine (A 250 µM) in free serum medium and then exposed (24 h) to TNF-α (T 60 ng/mL). Cell viability measured by the MTT assay and expressed as OD was: C 1.10±0.17; *HAT 0.43±0.18; *P