N-acetylcysteine, an antioxidant with anti-adipogenic effect on adipocytes

PIERALISI A; SOTO D; GABRIELLI M; MARTINI, C; DIZ V; ROVNER F; VILA MC; CALVO JC; GUERRA LN

Berlin

Congreso; OCC World Congress 2017 and Annual SFRR-E Conference; 2017

Reports about antioxidant effect in obesity are contradictory. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during adipocyte differentiation, through the inhibition of adipogenic transcriptor factors expression, such as PPARγ and, MAPKs phosphorylation (Pieralisi et al,Redox Biol 2016; Soto el al,Redox Rep 2016). Here we evaluated NAC on fully differentiated cells (3T3-L1 adipocytes: AC). Treatments with 0.01 to 5 mM NAC, included in culture media for 5 days, were not toxic. 5mM NAC treatment on AC (ACN) provoked a decrease of 60% in cellular Triglycerides (Tg) content (1.22+0.09 gTg/g protein [AC] vs 0.49+0.03 gTg/g protein [ACN], p