IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Photodamage induces mitochondrial quality control dysregulation in retinal pigment epithelium. Implications for age-related macular degeneration
Autor/es:
ALAIMO A; GARCIA LIÑARES G; BUJJAMER JM; PORTE ALCON S; GOROJOD R. M.; GRECCO HE; BALDESSARI A; KOTLER M.L
Lugar:
Ciudad Autonoma de Buenos Aires
Reunión:
Jornada; Jornadas Interdisciplinarias de Química Biológica; 2017
Institución organizadora:
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Resumen:
Age-related macular degeneration (AMD) is a late-onset neurodegenerative retinal disease. Accumulated lipofuscin fluorophore A2E in retinal pigment epithelium (RPE) confers susceptibility to blue light-mediated damage, a risk factor for AMD. Cells have developed well-coordinated Mitochondrial Quality Control (MQC) mechanisms in order to limit mitochondrial injury induced by oxidative stress, ensure mitochondrial integrity and preserve cellular homeostasis. RPE dysfunction is a critical event in AMD. Thus, our study was designed to evaluate MQC status, including mitochondrial dynamics (fusion/fission) and mitophagy, under photooxidative damage conditions in RPE.Light-emitting diodes irradiation (λ=445nm; 1.7mW/cm2, 30min) significantly reduced the viability of both non-pigmented and 10 or 25μM A2E-containing human RPE cells (MTT assay: 52%, 46%, 31%, respectively) as well as increased mitochondrial anion superoxide production (MitoSOX). A2E (25µM), along with blue light, induced apoptosis measured by Annexin V/PI-flow cytometry (6-fold, p