IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ERYTHROPOIETIN-INDUCED ENDOTHELIAL CELL MIGRATION RELIES ON AN INTRACELLULAR CALCIUM INCREASE
Autor/es:
CHAMORRO, ME; VITTORI, D; MALTANERI, R; NESSE, A; SCHIAPPACASSE, A
Reunión:
Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016
Resumen:
Apart from its effect on the hematopoietic system, erythropoietin (Epo), a glycosylated cytokine produced by the adult kidney, has also been involved in angiogenesis, a process with important physiological and pathological implications. Since the mechanisms underlying such proangiogenic behavior are little understood, it was our purpose to study possible effectors of Epo in cultures of the endothelial cell line EA.hy926, with special interest in the comparison with its non-hematopoietic carbamylated derivative (cEpo).Calcium (Ca) plays a key role in cell migration, through regulation of focal adhesions and cytoskeleton dynamics. In wound-healing assays, Epo-induced migration (200 ng/mL, 15 h) was inhibited by incubation with the Ca2+ chelator EGTA (1 mM) (Control: 27±2.0%; *Epo: 35±0.9%; Epo+EGTA: 27±2.1%; *P