IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TRANSCRIPTOMIC PROFILING OF EPITHELIAL TO MESENCHYMAL TRANSITION BIOMARKERS UNDER HEME-OXYGENASE 1 (HO-1) MODULATION IN PROSTATE CANCER
Autor/es:
LAGE VICKERS, SOFIA; ANSELMINO, NICOLAS; VAZQUEZ, ELBA; SCHUSTER, FEDERICO; ORTIZ, EMILIANO; GUERON, GERALDINE; PAEZ, ALEJANDRA; COTIGNOLA, JAVIER
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2016
Institución organizadora:
Sociedad Argentina de Investigacion Clinica
Resumen:
Epithelial?mesenchymal transition (EMT) is a cellular mechanism long recognized as a central feature of normal development and cancer. During tumorigenesis EMT may increase the motility and invasiveness of cancer cells. This process plays critical roles in the development of the prostate gland. Loss of epithelial markers (e.g. E-cadherin) and gain of mesenchymal markers (e.g N-cadherin) at the leading edge of solid tumors are associated with progression to metastasis. Elevated N-Cadherin has been shown to be a significant predictor of clinical recurrence in prostate cancer patients following radical prostatectomy. We have previously described the anti-tumoral effect of heme oxygenase I (HO-1) in PCa. To assess EMT differential expression in PCa cells overexpressing HO-1 pharmacologically (hemin treatment) or genetically (transfected with pcDNA3HO-1 vector) we performed RNAseq on PC3HO-1 vs. PC3pcDNA3 (empty vector) and PC3 hemin vs. PC3 control. For both comparisons, we obtained putative differential subsets of up-regulated (≥2, ≥3, ≥5, ≥8 fold change cut-off) and down-regulated (≤-2, ≤-3, ≤-5, ≤-8 fold change cut-off) genes. We screened for overlapping up or down regulated genes on same threshold subsets. Results show 92 down (≤-2, p