ENHANCEMENT OF GLUCOCORTICOID RECEPTOR TRANSCRIPTIONAL ACTIVITY BY CB1 CANNABINOID RECEPTOR SIGNALING. ITS ROLE ON MEMORY CONSOLIDATION OF AVERSIVE EXPERIENCES

DOMÍNGUEZ RUBIO, AP; FITZSIMONS, CP; ZAPPIA, CD; FRANCHI, AM; GRANJA GALEANO, G; ZORRILLA ZUBILETE, MA; MONCZOR, F

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC),; 2016

The cannabinoid receptor type 1 (CB )and the glucocorticoidreceptor (GR) are coexpressed in several brain areas involved inlearning and memory, including the hippocampus, a brain areainvolved in spatial and episodic memory. glucocorticoids mediatethe behavioral consequences of stress, such as memory consolidation associated with emotionally arousing events. The downstreamimmediate-early gene Egr1 is a GR-responsive gene involved inmemory consolidation. It has been suggested that CB1 signalingmodulates GR-mediated transcriptional activity. Here, we studiedthe effect of CB signaling on GR-mediated transcriptional activityrelated to memory consolidation of aversive events. For in-vitroudies, we used the HT22 neuronal cell line, derived from murinehippocampus. In these cells, induction of Egr1 by the GR agonistDexamethasone (Dex) was potentiated by the selective CB 1 agonistmeta-anandamide (300%, p