Cytotoxicity assessment of hydroxyapatite nanoparticles in normal and tumor cell lines

PORTE ALCON, S.; KOTLER, M. L.; GOROJOD, R. M.; DITTLER, M. L.; ALAIMO, A.; GONZALEZ, M. C.

Mar del Plata

Congreso; LXI Reunión de la Sociedad Argentina de Investigación Clínica; 2016

ARGENTINE SOCIETY FOR CLINICAL INVESTIGATION (SAIC)

Hydroxyapatite (Ca10(PO4)6(OH)2; HAP) is an essential component of the human bone inorganic phase. NanoscaledHAP (nHAP) presents emergent properties in comparison to its bulk counterpart, including an inhibitory effect on the proliferation of some types of tumor cells. However, little is known regarding to the potential adverse effects on human health. In this study, we aimed to determine the cytotoxicity of nHAP in both normal and tumor cell lines. For this purpose, we evaluated cell viability (MTT assay) after exposure to nHAP (50-200μg/ml, 24-72h) in both normal (fibroblasts NIH/3T3, microglia BV2 and retinal pigment epithelial cells ARPE-19) and tumor cell lines (astroglia C6, liver HepG2, lung A549 and epithelial HEp-2). Some cellular lines were selected for additional evaluation: lysosomal and nuclear integrity (LysotrackerRed DND-99 and Hoechst 33258 staining, respectively) and cell proliferation by clonogenicassay and Ki67 immunolabeling. Data obtained demonstrated a cytotoxiceffect in C6 and HEp-2 cells after 24h exposure (C6: 100μg/ml: 14±5% p