Association of the IL-17A rs2275913 single nucleotide polymorphism with tuberculosis severity in Argentina

PELLEGRINI JOAQUIN M; CASCO NICOLAS; PALMERO DOMINGO J; ROLANDELLI AGUSTIN; TATEOSIAN NANCY L; LEVI ALBERTO; GARCÍA VERÓNICA E; HERNANDEZ DEL PINO RODRIGO; AMIANO NICOLAS O; GUTIERREZ MARISA

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigaciones Clinicas (SAIC), LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI) y XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

Mycobacterium tuberculosis (Mtb) causes nearly 10 millions of new tuberculosis disease cases annually. However, most individuals exposed to Mtb do not develop tuberculosis, suggesting the influence of a human genetic component. Previously, we investigated the association of the rs2275913 SNP (G->A) from IL-17A and tuberculosis in Argentina, and found an association between the AA genotype and tuberculosis resistance. In this work, we evaluated the functional relevance of this SNP during the immune response of the host against Mtb and analyzed its impact on clinical parameters of the disease severity. Thus, peripheral blood mononuclear cells (PBMCs) from GG, GA and AA healthy donors (HD) and tuberculosis patients (TB) were obtained and stimulated for five days with a Mtb lysate (Mtb-Ag), and IL-17A and IFN-gamma production were measured by ELISA and Flow Cytometry. Our findings indicated that, within the HD population, AA cells produced the highest amounts of IL-17A (p