CONICET | Buscador de Institutos y Recursos Humanos

rostate cancer Ca is the second leading cause of death by cancer in males orld ide. A chronic in ammatory microen- vironment is a decisive factor of Ca progression. The induction of H represents an essential event in cellular responses to pro oxidative and pro in ammatory insults maintaining cellular homeostasis. H has been proposed to act as a biosensor regu- lating cell destination. e previously reported by Ch analysis that H associates ith gene promoters relevant in Ca SA, u A and . These results provide a novel function for H do n modulating A transcriptional activity. e have also sho n that H physically interacts ith transcription factors TF such as ST A T and F . T o screen for potential H TF partners, e performed Aseq on Ca cells overexpressing H phar- macologically hemin treatment or genetically transfected ith pc A H vector . ioinformatics analyses ere performed in order to identify putative TF binding motifs in promoter sequences of H up or do n regulated genes. The regulatory regions of up or do n regulated genes ere considered for analysis b up and . b do nstream of TSS . e established a or ing pipeline here multiple bioinformatics tools ere applied to obtain the regulatory regions E E suite . A list of TF motifs over represented in these regulatory region datasets compared to a neutral one regulatory regions of genes ith . fold regula- tion . as obtained by Analysis of otif Enrichment tool A E . esults rendered lists of TF associated ith each data set. The same analysis as done for the overlapping H modulated genes pharmacologically and genetically revealing t o ey TFs relevant in Ca component of the A transcription factor and T F , co activator of the androgen receptor. verall, our results provide further evidence for a novel function for H as a potential co regulator of transcription in Ca.