IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Postnatal development of rut pups after maternal low-dose exposure to the neonicotinoid insecticida acetamiprid during gestation
Autor/es:
RUEDA, L.; KRISTOFF, G.; KANDEL GAMBARTE, P.; ROVEDATTI, M. G.; FUCHS, J.; WOLANSKY, M. J.
Reunión:
Congreso; XIV Internacional Congress of Toxicology; 2016
Resumen:
Neonicotinoids are modern pesticides mostly used in agricultural pest control. While ~20 years ago NNTs were proposed to be highly selective for target species and considered safe for humans, more recent developmental toxicology (DT) studies have raised safety concerns regarding exposure during gestation and early infancy. As part of the pesticide mixture toxicology studies we are conducting to assess the joint action of low levels of NNTs and other modern pesticides, here we show preliminary results of a DT assessment of the NNT compound acetamiprid (ACP) in Wistar rats. Methods: We selected two exposure levels: 1 and 6 mg/kg/day. These daily doses were both below the reported NOAELs for maternal and developmental toxicity of ACP in rats, i.e., 16 mg/kg/day (USEPA), but quite above its ADI of 0.07 mg/kg/day. ACP was dissolved in the water offered ad libitum to the pregnant dams from gestational day (GD) 5 to 20. The study was divided into blocks, and 4-6 litters per treatment have been so far evaluated. Dams and their male and female offspring were monitored for body weight gain, developmental landmarks such as eye opening, fur appearance, and anogenital distance. Last, experimental pups were evaluated using a 7-session long, associativemotor test (Circling Training Test, CTT) at late adolescence to explore potential enduring effects of gestational treatments on fine nervous system function. Results: No evident alteration was observed in the dams or the offspring in any case. Nevertheless, ACP treated pups showed trends for a dose-related decrease in body weight and a poorer performance through the first sessions of the associative-spatial conditioning in the CTT test. Animals were sacrificed soon after the CTT assays, and various target tissues (brain, kidney and gonads) were dissected out for determining organ/body weight ratios (work in progress).