Amniotic membrane conditioned medium promotes cell death and inhibits proliferation of hepatocarcinoma HepG2 cells

BERNARDO MASKIN; VICTOR SÁNCHEZ MARGALET; RODRIGO RIEDEL; MARIANA JAIME; CECILIA VARONE; ANTONIO PÉREZ-PÉREZ; ORNELLA PAROLINI; JULIETA MAYMÓ

Puerto Varas

Congreso; VI Simposio Latinoamericano Interacción Materno-fetal y Placenta (SLIMP); 2017

SLIMP

The placenta and fetal membranes have recently been proposed as animportant stem cells source for regenerative medicine. Stem cells derivedfrom amniotic membrane offer considerable advantages over other stemcells. Not only are amnion-derived stem cells applicable in regenerativemedicine, but also have antitumoral properties. Hepatic failure is one ofthe major cause of morbidity and mortality, and despite the developmentin therapies, hepatocarcinoma rates are high worldwide. Little is knownabout the mechanisms involved in the antitumoral effect of the amnioticmembrane and their cells.Objectives: The aim of this work was to study some aspects of cell deathand proliferation induced by amniotic membrane conditioned medium(AM-CM) in hepatocarcinoma cells.Methods: We analysed the expression of proapoptotic proteins (p53,Caspase-3, PARP-1, p21) by qRT-PCR and Western blot, in HepG2 cellstreated with AM-CM. We also studied cell proliferation by [3H]thymidineincorporation assay and cell viability by MTT assay. Serum deprivation wasused as cell death control.Results: We found a significant increase (P