IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of Argentine Chitosan for Drug Delivery
Autor/es:
JIMENA H MARTÍNEZ; FACUNDO LÓPEZ SIERRA ; OSCAR E PÉREZ; FEDERICO COLUCCIO-LESKOW
Lugar:
Buenos Aires
Reunión:
Simposio; Frontiers in Physical Sciences; 2016
Institución organizadora:
Centro de Latinoamericano de Formación Interdisciplinaria y MinCyT.
Resumen:
Chitosan (CHI), the deacetylated form of Chitin, is a cationic polysaccharide with peculiar properties due to the primary amines all along its carbonated skeleton. Its antibiotic, biocompatible and biodegradable properties and its capacity to interact with anionic polymers and the extracellular matrix make it a good candidate for drug delivery. In Argentina, shrimp industries produce considerable amounts of chitin. It is necessary to characterize the properties of the CHI derived from this byproduct in order to scale up the production and take advantage of its commercial and pharmacological use. CHI has been previously used to encapsulate bioactives into nanoparticles as delivery systems. Nanocapsules are thought to protect and to progressively liberate ligands of high biomedical impact, such as growth factors. These cell growth promoters require site specific and time controllled release in order to best perform and to avoid side effects. CHI nanocarriers would allow to improve tissue regeneration therapies and serve as supplies for tissue engineering.In the present study we calculated the viscous molecular weight of CHI produced in laboratory conditions (NanoSano), and its effect on the viability of epithelial HeLa cells and 3T3 fibroblasts.Using an Ubelhode viscometer and according to the Mark-Houwink-Sakurada equation (constants: K=1.81x10?3 and α= 0.93) we found our CHI to be 3.56x105 Da. Cell viability was evaluated by MTT with different concentrations of CHI every 24 hours for three days. CHI has shown to have no significant effect on HeLa viability (n=12; p