IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Leptin protects trophoblastic cells from UV radiation-induced apoptosis
Autor/es:
PEREZ PEREZ, ANTONIO; VARONE, CECILIA; MASKIN, BERNARDO; TORO, AYELEN; SANCHEZ-MARGALET, VICTOR
Lugar:
Mar del Plata
Reunión:
Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016
Resumen:
It is well established that leptin modulates angiogenesis, growth, immunomodulation as well as anti-apoptotic effects in the placenta. In recent studies we demonstrated that leptin promotes survival of placental cells involving the regulation of different intrinsic pathway factors under stress conditions such as serum deprivation, hyperthermia and acidosis. Moreover we also proved that leptin exerts a negative regulatory effect on p53 levels. In this context we aimed to study leptin effect on trophoblastic cells exposed to UV radiation since p53 plays a pivotal role in UV damage response. Swan-71 cells were exposed to UV radiation (1, 2 and 4 mJ/cm2) and treated with or without recombinant leptin (50, 100 and 250 ng/ml) during 5 h. Western blot were carried out to evaluate leptin action on anti- and pro-apoptotic intermediaries. We demonstrated that p53 expression was increased by UV exposure (p