IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Intrinsic role of Galectin-1 in the control of the functional properties of immune cells in a prostate cancer context
Autor/es:
ENRIQUE SEBASTIÁN CORAPI, GUSTAVO EZEQUIEL CARRIZO, LAURA GIRIBALDI, FELIPE MARTÍN JAWORSKI, GABRIEL ADRIÁN RABINOVICH, DANIEL COMPAGNO & DIEGO LADERACH
Reunión:
Exposicin; Reunión Anual Sociedad Argentina de Inmunología 2016; 2016
Resumen:
The identification of effective new therapies for prostate cancer (PCa) requires a better understanding of the multiple molecular interactions between tumour cells and their associated stroma.In this context, Galectin-1 (Gal-1) plays a major role in determining the properties of the prostatic carcinoma microenvironment. The aim of this study is to elucídate whether Gal-1, in additionto promote tumor neoangiogenesis and immune regulations such as induction of apoptosis on activated T cells, inefficient antigen presentation and expansion of regulatory T cells, playsan additional role as an intrinsic regulator of CD8+ T cell functional properties. To address this concern, we used an in vitro T cell polyclonal activation model combining different cell types (purified by cell sorting) in a prostate tumor microenvironment. Thus, by combination of Gal-1 deficient (Lgals1-/-) and wild type (WT) cells, we were able to assess how the endogenous Gal-1 of each cellularcompartment impacts on the CD8+ T cell properties (proliferation and citotoxicity). The absence of Gal-1 in antigen presenting cells (APCs) did not significantly modifythe proliferative properties of CD8+T cells. Conversely, the absence of Gal-1 in CD4+ T cells induced a 1.21 fold increase in the proliferation of CD8+T cells. However, the most significant difference in the proliferation was obtained by absence of intrinsic Gal-1 in the CD8+ T cells themselves (2.12). Taking into account Gal-1 controls T cell proliferation, we further evaluated whether Gal-1is relevant in controlling effector function. Our results demonstrated that upon activation, Lgals1-/- T cells have increased ability to degranulate (evaluatedas % (1.87fold) and the content of granules (2.48 fold increase) (p