IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
STUDY OF THE TWO UDPGLUCOSE-GLYCOPROTEIN GLUCOSYLTRANSFERASE (UGGT) HOMOLOGUES IN A MOUSE MODEL OF INTESTINAL INFLAMMATION
Autor/es:
BOTTERO E.,; RIAL HAWILA M. R; ACOSTA MONTALVO ANA GABRIELA; ROUX M.E; CASTRO O. A; MIRANDA S.E.
Lugar:
Capital federal
Reunión:
Congreso; SAI 2015; 2015
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Background: Intestinal epithelial cells (IEC) are particularly susceptible to endoplasmic reticulum stress. UPR (unfolded protein response) is required to maintain IEC homeostasis and its impairment can lead to inflammation as observed in Crohn´s disease. UGGT is the key component of the quality control mechanism of glycoprotein folding. We have previously described a second isoform in mouse and C. elegans. In this study, we explored UGGTs expression in mouse small intestine and their modulation by accoustic stimuli (AS), a model of Crohn´s disease.Methods: CBA/J mice were exposed to 24h AS (300Hz-70dB) and randomized (n=5). Mice were killed: 1) after AS; 2) three weeks after AS; 3) mice were submitted to AS once a week during a month and then were killed. Control groups did not receive AS. UGGT1/UGGT2 expression was analyzed in tissue sections by immunohistochemical analysis. To this aim, antiserum anti-UGGT2 was raised in rabbits employing a multiantigenic peptide synthesized with a sequence of UGGT2 (NP_001074721.2) not present in UGGT1 (NP_942602.2). The specificity was confirmed by western blot.Results: UGGTs are moderately expressed in normal conditions. After one (1) or various AS (3), UGGT1/UGGT2 expression increased proportionally to inflammation (for both: group 3>1, p