HUMAN TROPHOBLAST CELL MIGRATION AND INVASION ARE INDUCED BY ENDOGENOUS VASOACTIVE INTESTINAL PEPTIDE (VIP) THROUGH AUTOCRINE CIRCUITS AND VPAC2 OVEREXPRESSION

DAIANA VOTA; VANESA HAUK; AYELEN TORO; ESTEBAN GRASSO; DANIEL PAPARINI; ROSANNA RAMHORST; CECILIA VARONE; CLAUDIA PÉREZ LEIRÓS

Mar del Plata

Simposio; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta (VI SLIMP) and the V Latin American Symposium on Reproductive Immunology (V LASRI); 2015

The generation of the human maternal-placental interface requires trophoblast differentiation into phenotypes able to migrate and invade the deciduas. VIP is a pleiotropic polypeptide with immunomodulatory effects through VPAC1 and VPAC2 receptors. Previously, we have reported on its immune homeostasis maintenance role at early pregnancy. The expression of VIP in various cell types is induced by growth factors or neurotransmitters and depends on CRE (cAMP responsive elements) and gp130 cytokine (CyRE) sites on its promoter. Objective: To investigate VIP/VPAC2 expression on first trimester cytotrophoblast cells (Swan-71; HTR-8) and its impact on trophoblast cell migration and invasion. Methods: VIP expression was measured by flow cytometry and RT-qPCR; cell migration was determined by wound healing assays and invasiveness in Matrigel-covered transwells. Trophoblast cells were transfected with XtremeGENE HPDNA reagent with VPAC2 or CRE-Luciferase plasmids for 24h prior to the stimuli, normalized to GFP or beta-gal. Results: Endogenous VIP expression was detected in trophoblast cells and its synthesis was induced after 18 h incubation with 10nM VIP (P