VIP PROMOTES APOPTOTIC CELL PHAGOCYTOSIS BY MONOCYTES/MACROPHAGES THROUGH αvß3 INTEGRIN-THROMBOSPONDIN PORTAL FORMATION AND ENHANCED TROPHOBLAST MIGRATION IN A MODEL OF MATERNAL-PLACENTAL INTERACTION

DANIEL PAPARINI; DAIANA VOTA; ESTEBAN GRASSO; VANESA HAUK; GUILLERMINA CALO; ROSANNA RAMHORST; CLAUDIA PEREZ LEIROS

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Society for Immunology Meeting II French-Argentinean Immunology Meeting; 2015

Background.Homeostasis maintenance at the early maternal-fetal interface requires active tissue remodelling processes and the clearance of apoptotic cells by macrophages in an immunosuppressant microenvironment. Vasoactive intestinal peptide (VIP) is a pleiotropic polypeptide synthesized by trophoblast cells that induces an anti-inflammatory profile in monocytes and macrophages (Mo/Ma). Here we explored VIP effect on trophoblast-monocyte interaction with special focus on Mo phagocytosis of apoptotic cells and trophoblast cell wound healing.Methods.Mo were purified by Percoll from PBMC of healthy volunteers and cultured in the presence/absence of VIP (1-100nM) or with conditioned media (CM)from first trimester human cytotrophoblast cell line (Swan-71)(Tb) treated or not with VIP (CM-VIP). Apoptosis was induced in Tb with camptothecin. CFSE-labelled apoptotic cell phagocytosis by CD14+ cellsand αvß3 expression was assessed by flow cytometry and confocal microscopy. Thrombospondin1 expression was determined by RT-qPCR.Wound healingassays were run in trophoblast monolayers.Results.CM from Tb cells enhanced phagocytosis of apoptotic cells and induced αvß3 integrin relocalization and expressionon Mo/Mavs. DMEM(frecuency % X±SE: 77.1±2.0 vs. 49.6±1.5; P