IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
VIP induces human trophoblast cell migration and invasion through VPAC2 receptors and autocrine circuits involving cAMP/PKA/CRE signaling
Autor/es:
DAIANA VOTA; VANESA HAUK; AYELÉN TORO; DANIEL PAPARINI; FÁTIMA MERECH; CECILIA VARONE; ROSANNA RAMHORST; CLAUDIA PÉREZ LEIRÓS
Lugar:
Cappadocia
Reunión:
Congreso; 12th International Symposium on VIP, PACAP and Related Peptides; 2015
Resumen:
The generation of the human maternal-placental interface involves the differentiation of trophoblast cells to phenotypes suitable for migration and invasion of the deciduas. We have proposed that VIP contributes to immune homeostasis maintenance at early pregnancy. Growth factors and neurotransmitters induce VIP expression in various cell types and depends on CRE (cAMP responsive elements) and gp130 cytokine (CyRE) sites on its promoter. Our goal was to explore VIP/VPAC2 expression on first trimester human trophoblast cells (lines Swan71 and HTR8) and the impact of VPAC2 over-expression or VIP silencing on trophoblast cell function and CRE-mediated signaling. VIP expression was measured by flow cytometry and RT-qPCR; cell migration by wound healing assays and invasiveness on Matrigel-covered transwells. Trophoblast cells were transfected (X-tremeGENE HPDNA reagent) with VPAC2 or CRE-Luciferase plasmids for 24h prior to stimuli, normalized to GFP or b-gal. VIP silencing was carried out for 72 h using VIP siRNA or an irrelevant siRNA as negative control. Swan71 cells express VIP and its synthesis was induced by 10nM VIP (p