IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Leptin is involved in human trophoblastic migration and invasion
Autor/es:
AYELÉN TORO; ROCÍO SAMPAYO; ANTONIO PÉREZ PÉREZ; BERNARDO MASKIN; VÍCTOR SÁNCHEZ MARGALET; MARINA SIMIAN; CECILIA L. VARONE
Reunión:
Simposio; VI SLIMP- Latin American Symposium on Maternal Fetal Interaction & Placenta; 2015
Resumen:
BACKGROUNDCytotrophoblastic (CTB) cells of the human placenta proliferate, migrate and invade the uterus during pregnancy to allow implantation and placentation. To successfully become invasive the CTB cells undergo a transition from epithelial to highly invasive mesenchymal phenotype, called EMT (epithelial-mesenchymal transition). EMT is characterized by up-regulation of motility promoting molecules such as the adhesion molecule β1 Integrin and the loss of epithelial markers such as E-cadherin. Leptin is produced by placenta and we have previously demonstrated that promotes proliferation and survival of trophoblastic cells. OBJECTIVEWe aimed to study a possible role of leptin in cell migration and invasion by evaluating its effect on β1 Integrin and E-cadherin expression. METHODSThe human CTB cell line from first trimester Swan-71 was used. Cells were treated with different leptin concentrations (50, 100 and 250 ng/ml) for 48h. To study if leptin effect was reversible, after leptin treatment, media was replaced with fresh medium for 24h. We also investigated if metalloproteinases (MMPs) were necessary for leptin action. For this aim, we cultured Swan-71 cells were cultured with a MMPs inhibitor (GM6001, 10µM) during 2h before leptin treatment. In all the cases we evaluated β1 Integrin and E-cadherin expression by qRT-PCR or/and WB assays. To evaluate leptin effect on cell migration we performed the wound healing assays.RESULTSLeptin reduced E-cadherin expression at mRNA and protein levels. This effect was reversible and MMPs activity was necessary. However, leptin induced up-regulation of β1 Integrin expression at protein level without modifying mRNA level. Leptin action on β1 Integrin was reversible when cells were pretreated with 50 and 100 ng/ml of leptin. On the other hand, wound healing assay demonstrated that 100 ng/ml of leptin promotes cell migration.CONCLUSIONSAll these findings suggest that leptin promotes cell migration and invasiveness of trophoblastic cells.