IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PATHOGEN EXCLUSION AND IMMUNOMODULATORY EFFECT OF S-LAYER PROTEIN OFLACTOBACILLUS ACIDOPHILUS
Autor/es:
JOAQUINA FINA MARTIN; PRADO ACOSTA, MARIANO; RUZAL, SANDRA M.; BARQUERO, ANDREA
Lugar:
Mar del Plata
Reunión:
Congreso; SAIB 2015; 2015
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)
Resumen:
Lactobacilli are found in human intestine and some strains are consideredprobiotic. Surface layer (S-layer) protein constitute in some species their outermostcell envelope and have been considered to be involved in modulating the hostimmune system and inhibit pathogenic bacteria adhesion which has recentlygained attention as a way to prevent infection. Here, we investigate L.acidophilus ATCC4356 adherence to epithelial cell linesand ability to interfere with Pseudomonas aeruginosa by plate counts. The S-layermodulation of cytokines production in the murine macrophage cell line J774A.1and Caco-2 cells was also studied by ELISA and qRT-PCR.Interaction with mucins, immobilized on a PVDF membrane was assayed.Lactobacilli showed lower adherence to lung A549 cells than intestinal Caco-2cells ranging from 0.5 to 10% depending on the number of input bacteria and day-old Caco-2 monolayers. We found that S-layer protein was also able to producepathogen exclusion against Pseudomas when added purified; however, livelactobacilli with or without S-layer did not. Moreover, in macrophages co-incubatedfor 24 h with purified S-layer, IL-6 and TNF-α secretion was significantly increased.Likewise, purified S-layer led to an elevated expression peak of TNF-α; at 4h ofincubation in Caco-2 cells. Interaction with mucins was detected with both proteinand cells.