Role of VIP in the human decidualization program and contribution to immune profile conditioning

E. GRASSO, S.GORI, D. PAPARINI, G. SALOMONE, G. MARTINEZ, M. IRIGOYEN, C. RUHLMANN, C. PÉREZ LEIRÓS AND R. RAMHORST.

Cappadocia

Simposio; 12th Internatinal Symposium of VIP, VPAC and related pepetides; 2015

12th Internatinal Symposium of VIP, VPAC and related pepetides

The decidualization program involves phenotypic and functional shaping of endometrial cells for the proper attachment and invasion of the blastocyst. Progesterone action and intracellular cAMP levels are central to this process. Mediators such as cytokines, chemokines and growth factors are modulated to encompass decidualization. On the knowledge that vasoactive intestinal peptide (VIP) is produced by endometrial stromal cells and targets multiple circuits to promote an immunetolerant microenvironment, here we explored VIP contribution to the decidualization program and its effect on dendritic cell (CD) immune profile. Human endometrial stromal cell line (hESC) was cultured with/without VIP or MPA+dbcAMP. VIP/VPAC system and decidualization markers were evaluated by RTPCR and ELISA. Blastocyst-like spheroids (BLS) were obtained from Swan71cells and cultured on hESC monolayer. Monocytes were isolated from PBMCs, differenciated to DC and studied by FACS. MPA+dbcAMP decidualization increased VIP expression and secretion in hESC (p