IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genomic analysis of Butanol production in a novel thermophilic butanol producer
Autor/es:
DÍAZ PEÑA ROCÍO; MÉNDEZ BEATRIZ S; PETTINARI, M. JULIA
Lugar:
Córdoba
Reunión:
Congreso; XI Congreso Argentino de Microbiología General; 2015
Resumen:
Genomic analysis of Butanol production in anovel thermophilic butanol producer. Díaz Peña R, Méndez,B.S., and Pettinari MJ,IQUIBICEN-Departamentode Química Biológica, FCEyN-UBA,  Over the past decades, development ofbiofuels has raised a great interest as an alternative to fossil fuels whichgenerate large amounts CO2, emissions and toxic byproducts, (causativeagents of greenhouse effect), and are non-renewable resources. In this context,butanol has attracted special attention due to its advantages over ethanol. Notonly it is capable of producing more energy if properly harnessed but is also lesscorrosive and watersoluble, so it can be used in blending with gasoline ordiesel. Moreover its use does not require modifications to existing engines, ithas a lower vapor pressure and is less hygroscopicthan ethanol, acharacteristic that enables its transportation without altering the product. The microbial production of butanol by bacteriaof the genusClostridiumhas been studiedin detail. Such is the case of C. acetobutylicum, which has beenused inindustrial processes for the synthesis of acetone and butanol from differentsubstrates. In our laboratory we haveisolated and characterized a new species, originallynamed C.thermopapyrolyticum, an anaerobicthermopilic bacterium that is capable of producing butanol once it has reachedstationary state.To complete the genetic characterization ofthis microorganisms its genome has been sequenced. Genome to genome comparisonswith previously sequenced bacteria revealed that it is most probably a memberof the genus Thermoanaerobacterium,and not Clostridium. In view of this,we denominated it as Thermoanaeobacteriumsp. GCU5.To identify genes encoding the key enzymes in the metabolic pathway of n-butanol production,weperformed a genomic analysis using RAST annotation Server and Blast algorithm.Eightgenes involved in this pathwaywere identified: thl, hbd, crt, bcd,etfA, etfB, adh, bdh.It was observed that the genes thl, hbd, crt, bcd,etfA, etfB are organized in the operonbcs,whereas in C.acetobutylicumthe gene thl is not part of this operon.Furthermore, weobserved that Thermoanaeobacteriumsp. GCU5presents genes coding for enzymes involved in the metabolism of xylan (hemicellulose main component), cellobiose and sucrose, all components present on sugar cane agroindustrial residue.