IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IMMUNE HOMEOSTASIS DURING THE DECIDUALIZATION PROGRAM: PARTICIPATION OF VIP AND DENDRITIC CELLS
Autor/es:
ESTEBAN GRASSO; SOLEDAD GORI; DANIEL PAPARINI; LUCILA GALLINO; ELIZABETH SOCZEWSKI; GABRIELA SALAMONE; CLAUDIA PÉREZ LEIRÓS; ROSANNA RAMHORST
Lugar:
Mar del Plata
Reunión:
Simposio; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta (VI SLIMP) and the V Latin American Symposium on Reproductive Immunology (V LASRI); 2015
Resumen:
The decidualization program involves phenotype and functional changes in endometrial cells such as the production of different mediators that will facilitate the attachment and invasion of the blastocyst. Particularly, the vasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrial stromal cells among others, which displays multiple target circuits that allow immunotolerance. Objective: We investigated VIP contribution to the decidualization program and whether this process modulates the profile of dendritic cells (DC). Methods: A human endometrial stromal cell line (hESC) was evaluated. These cells were differentiated in the presence of VIP (10-7M) or medroxyprogesterone (MPA) and dbcAMP (Positive control) for 8 days. Phenotypic markers of decidualization, VIP and their receptors were quantified by RT-PCR. DC were obtained from monocytes isolated from PBMCs from fertile women and differentiated with IL-4 and GM-CSF. VIP and IL-10 secretion were quantified by ELISA. Results: When decidualization was induced by VIP, we observed an increase in characteristic markers such as IGFBP1, PRL and the KLF13/KLF9 ratio, IL-8 and SDF1 in a concentration-dependent manner (p