IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Modulation of Autphagy by cytokines during human active tuberculosis
Autor/es:
PELLEGRINI JOAQUIN M; TATEOSIAN NANCY L; AMIANO NICOLAS O; ROLANDELLI AGUSTIN; HERNANDEZ DEL PINO RODRIGO; GUTIERREZ MARISA; PALMERO DOMINGO J; GARCIA VERÓNICA E
Reunión:
Congreso; IV Congreso de la Sociedad Latinoamericana de Inmunodeficiencias (LASID), LXIII Reunión de la Sociedad Argentina de Inmunología (SAI) y II Reunión FAIC (French-Argentinean Immunology Congress); 2015
Resumen:
BACKGROUND: Th1 responses are crucial in the hostdefense against Mycobacterium tuberculosis (Mtb) infection, butIFN-gamma alone is not sufficient to the complete bacterialeradication. Actually, Th17 cells have been associated with Mtbinfection. Given that cytokines may modulate positively andnegatively the autophagic response affecting mycobacterialsurvival, we investigated the role of IFN-gamma and IL-17A onautophagy during human active tuberculosis. METHODS:Peripheral blood mononuclear cells (PBMCs) from healthy donors(HD) and patients with active tuberculosis (TB) were cultivatedfor 16 h without stimulus to allow monocyte adherence. Cellswere then either infected with a RD1 deleted-Mtb-strain(MtbDeltaRD1) or MtbH37Rv pathogenic strain, or stimulatedwith sonicated Mtb (Mtb-Ag) ± IFN-gamma (1.8ng/ml) or IL -17A (10ng/ml) for 24 h. Afterwards, autophagy levels wereanalyzed by Flow cytometry and ImmunofluorescenceMicroscopy against LC3-IIB and IFN-gamma production wasmeasured by ELISA. RESULTS: Mtb-Ag stimulation increasedthe levels of LC3?IIB in monocytes of TB patients. Importantly,when these stimulated cells were treated with IFN-gamma or IL-17A for 24 h, the induced levels of autophagy were significantlyhigher (p