IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cell Wall Integrity (CWI), MAPK and PKA signaling cross-talk in Saccharomyces cerevisiae
Autor/es:
CAÑONERO, L; PAUTASSO, C; ROSSI, S
Reunión:
Congreso; LI Reunion SAIB; 2015
Resumen:
The signal transduction cascade CWI pathway in S.cerevisiae responds to cell wall stress signals like heat shock, through a family of cell surface sensors (WSC1-3). These sensors activate MAPK cascade that serve to amplify a small signal initiated at the cell surface. CWI pathway cross talks with other signaling pathways as the protein kinase A pathway. We analyzed the regulation of TPK1 (one of the catalytic subunit of PKA) expression during heat stress by CWI pathway. Using β-galactosidase reporter and quantification of mRNA by qRT-PCR we demonstrate that Wsc1, Wsc2 and Wsc3 receptors are not redundant for heat shock response. The involvement of MAPK kinase pathway in TPK1 expression regulation was demonstrated using different deletion strains. The results also show that other parallel pathway should be involved. PKA phosphorylates Slt2/Mpk1 kinase, the last component in the MAPK cascade, producing an inhibition in the signaling pathway. Slt2/Mpk1 is negatively regulated by Sdp1 phosphatase, whose transcription is regulated by Msn2/4 transcription factors during heat shock. The localization of Msn2-GFP under this stress was analyzed in WT and wsc1Δ, wsc3Δ strains, and showed to be different in the mutant strains in comparison with the wt strain. The results indicate that exists a crosstalk among MAPK and PKA pathway during heat shock