VIP induces the decidualization program on human endometrial stromal cells and conditions dendritic cells profile

GRASSO E, GORI S, PAPARINI D, SALOMONE G, MARTINEZ G, IRIGOYEN M, RUHLMANN C, PÉREZ LEIRÓS C, RAMHORST R

Lisboa

Simposio; 31st Annual Meeting ESHRE; 2015

ESRHE

Abstract title: (max 25) VIP induces the decidualization program on human endometrial stromal cells and conditions dendritic cells profile2. Study question: (max 50) To investigate VIP (vasoactive intestinal peptide) contribution into the decidualization program, from phenotypic and functional aspects and its effect on dendritic cells (CD) immune-profile. Here, we used an in vitro implantation model based in the co-culture of blastocyst-like spheroids from trophoblast cells cultured on hESC monolayer decidualized with VIP.3. Summary answer: (max 50)50Our results suggest that VIP contributes to the decidualization process on hESC cells inducing phenotypic markers and chemokines expression. From the functional aspect, VIP-decidualizated hESC cells allow the blastocyst-like spheroides invasion and condition Dendritic cells to a tolerogenic profile preventing the increase of coestimualtory molecules and inducing IL-10 secretion.4. What is known already: (max 75)The decidualization program involves phenotype and functional changes on endometrial cells that facilitate the attachment and invasion of the blastocyst. Although progesterone is a key hormone, the trigger of this process is an intracellular cAMP increase. The decidualization involves the modulation of different mediators such as cytokines, chemokines and growth factors. Particularlly, the vasoactive intestinal peptide (VIP) is a neuropeptide produced by endometrial stromal cells among others, and displays multiple target circuits that allow immunetolerance. 5. Study design, size, duration: (max 50) 6. Participants/materials, setting, methods: (max 50) Human endometrial stromal cell line (hESC) was cultured with/without VIP or MPA+dbcAMP. VIP/VPAC system and decidualization markers were evaluated by RTPCR and ELISA. Blastocyst-like spheroids (BLS) were obtained from Swan71cells and cultured on hESC monolayer. Monocytes were isolated from PBMCs, differenciated to DC and studied by FACS.7. Main results and the role of chance: (max 125) The MPA+dbcAMP decidualization increased VIP expression and secretion (p