?The Sp1transcription Factor is involved in the Regulation of Placental Leptin Expression?

MALENA SCHANTON ; AYELÉN TORO; JULIETA MAYMÓ; ANTONIO PÉREZ-PÉREZ; YESICA GAMBINO; BERNARDO MASKIN; VICTOR SÁNCHEZ MARGALET; CECILIA VARONE

Mar del Plata

Congreso; V Simposio Latinoamericano: Interacción Materno-fetal y Placenta (SLIMP); 2015

SLIMP

Objectives: Leptin is a key hormone in placental physiology. It regulatestrophoblast proliferation, inhibits apoptosis, stimulates protein synthesis,and regulates fetal growth and development. The mechanisms involved inthe regulation of placental leptin expression are not fully understood.Previous results from our lab demonstrated that estradiol (E2) regulatesleptin expression involving genomic and non-genomic effects. Sp1 is aubiquitous transcription factor. Several potential binding sites for Sp1 havebeen found in the leptin promoter. One of them is present in the regionidentified as PLE (enhancer of the placental leptin) involved in E2 effect. Inthe present study, we analyzed the effect of Sp1 in the induction of leptinexpression by E2 in human placental cells.Methods: BeWo cells were cultured under standard conditions. BeWo cellswere transiently transfected with different reporter vectors and expressionvectors for Sp1 or ERa.Results: We observed that Sp1 overexpression increased basal leptin promoteractivity (p