IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE OTHER SIDE OF COVID-19 PANDEMIC: EFFECTS ON FEMALE FERTILITY
Autor/es:
VELÁZQUEZ, CANDELA; DE ZÚÑIGA, IGNACIO; LAVOLPE, MARIANO; ABRAMOVICH, DALHIA; HERRERO, YAMILA; OUBIÑA, GONZALO; PEÑA, MARIANA GÓMEZ; VEIGA, FLORENCIA; SCOTTI, LEOPOLDINA; NATALIA PASCUALI; VANESA HAUK; MARTÍNEZ, GUSTAVO; NEUSPILLER, FERNANDO; FERNANDA PARBORELL
Lugar:
Mar del Plata
Reunión:
Congreso; Congreso de la Sociedad Argentina de Investigación Clínica 2021; 2021
Resumen:
SARS-CoV-2 invades the target cell by binding to angiotensin converting enzyme 2 (ACE-2). In the human ovary, ACE-2 is expressedin stromal and granulosa cells.Our objective was to evaluate the effect of SARS-CoV-2 infectionon female gonad.FF (follicular fluid) from patients undergoing ART (n= 80; 21?41years old; November 2020-April 2021) were divided in two groups:FF from control patients and FF from recovered COVID-19 patients(asymptomatic and with mild symptoms).The levels of IgG antibodies against SARS-CoV-2, IL-1β, IL-10 andVEGF were measured in FF by ELISA.Using a granulosa cell line (COV434) and an endothelial cell line(EA.hy926), we studied the effect of FF from control and recovered COVID-19 patients. The expression of StAR, ERα and ERβ,3β-HSD, VEGF, ANGPTs (angiogenesis-related proteins) andγH2AX (DNA damage marker) was evaluated by WB. Proliferationwas evaluated by a WST-1 assay. Endothelial cell migration wasevaluated by a wound healing assay. We performed Student?s t testor one-way ANOVA.The results showed that 91.3% of post-COVID-19 FF was positivefor IgG against SARS-CoV-2. Patients with higher levels of SARSCoV-2 IgG showed a decrease in the number of retrieved oocytes(p