EXPRESSION OF TRANSFERRIN RECEPTOR 2 IS REGULATED BY ERYTHROPOIETIN IN HEPG2 CELLS

VILLAROSA, JULIETA; MALTANERI, ROMINA; NESSE, ALCIRA; CHAMORRO, M. EUGENIA; VITTORI, DANIELA

modalidad virtual

Congreso; Reunión Anual de Biociencias; 2021

Sociedad Argentina de Investigación Clínica

The systemic regulation of Fe availability relies on the hepcidin/ferroportin axis, which controls the mobilization of Fe stores for different cellular functions. It is currently recognized that transferrin receptors (TfRs) not only play a role in Fe acquisition but also in modulating its availability. While TfR1 has a high affinity for holo-transferrin (Tf-Fe) ─with which it complexes and internalizes thus favoring cellular deposition of Fe─, TfR2 is deemed to regulate hepcidin expression, through mechanisms yet to be clarified. Erythropoiesis is a major Fe-consuming physiological process, although further research is needed to understand its effect on the different factors involved in Fe homeostasis. In previous studies we found an inhibitory effect of the erythroid survival factor erythropoietin (Epo, 160 ng/mL, 6 h) on hepcidin mRNA levels in HepG2 hepatocarcinoma cells. Here we report that Epo decreased TfR2 mRNA levels in this cell type in the same treatment period (real-time PCR, a.u.: Control=1.0, *Epo 6h=0.4±0.1, Epo 15h=1.3±0.3, *p