IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EXACERBATED ENDOPLASMIC RETICULUM STRESS SUFFERED BY ENDOMETRIAL STROMAL CELLS ALTERS THE CONDITIONING OF MATERNAL MONOCYTES TO A TOLEROGENIC DENDRITIC CELL PROFILE
Autor/es:
ELIZABETH SOCZEWSKI; LARA CASTAGNOLA ; CLAUDIA PÉREZ LEIRÓS ; ANA SCHAFIR; DAIANA VOTA ; JORGELINA BLEJER; SOLEDAD GORI ; LAURA FERNÁNDEZ; YESICA BESTACH ; ALEJANDRA GRASSI BASSINO ; ROSANNA RAMHORST
Lugar:
Congreso Virtual
Reunión:
Congreso; Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2021
Institución organizadora:
Sociedad Argentina de Inmunología (SAI)
Resumen:
Endometrial stromal cells undergo physiological endoplasmic reticulum stress (ERS) and unfolded protein response (UPR) allowing the secretion of immunoregulators and associated with a sterile inflammatory response to achieve a successful implantation. However, an exacerbated ERS/UPR was found in endometria of women with recurrent pregnancy loss, suggesting that a rigorous balance is required. Considering that stromal cells condition maternal monocytes to a tolerogenic dendritic cell profile, here we evaluated the impact of ERS/UPR triggered on stromal cells on monocyte differentiation. Thus, human endometrial stromal cell line (HESC) were treated: +/- Thapsigargin (an ERS-inducer Tg, 4h) or +/- decidualization stimuli (MPA+db-cAMP for 8 days). Conditioned media (CM) were collected after 48h. Then, isolated monocytes from peripheral blood mononuclear cells from healthy women were cultured with rhGM-CSF+rhIL-4 for 5 days in the absence/presence of CM. Monocyte-derived cultures differentiated with HESC+Tg CM showed a lower frequency of CD1a+CD14- cells compared with HESC CM, quantified by FACS analysis (p