IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
capítulos de libros
Título:
Fluoromycobacteriophages for drug susceptibility testing (DST) of Mycobacteria.
Autor/es:
MARIANA PIURI; GRAHAM F HATFULL
Libro:
Bacteriophages Methods and Protocols
Editorial:
Humana Press
Referencias:
Año: 2019; p. 27 - 36
Resumen:
Fluoromycobacteriophages are a new class of reporter phages that contain fluorescent reporter genes (gfp, ZsYellow and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.