Neutrophil autophagy during human active tuberculosis is modulated by SLAMF1

SABBIONE, FLORENCIA; CASTELLO, FLORENCIA ANDREA; LEVI, ALBERTO; TREVANI, ANALÍA SILVINA; MORELLI, MARÍA PAULA; AMIANO, NICOLÁS OSCAR; CIALLELLA, LORENA; GARCÍA, VERÓNICA EDITH; PELLEGRINI, JOAQUÍN MIGUEL; TATEOSIAN, NANCY LILIANA; PALMERO, DOMINGO; COLOMBO, MARÍA ISABEL

AUTOPHAGY

LANDES BIOSCIENCE

Año: 2020 p. 1 - 10

1554-8627

Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominate in tuberculosis patients? lungs. Neutrophils phagocytose the pathogen, but the mechanism of pathogen elimination is controversial. Macroautophagy/autophagy, a crucial mechanism for several neutrophil functions, can be modulated by immunological mediators. The costimulatory molecule SLAMF1 can act as a microbial sensor in macrophages being also able to interact with autophagy-related proteins. Here, we demonstrate for the first time that human neutrophils express SLAMF1 upon Mtb-stimulation. Furthermore, SLAMF1 was found colocalizing with LC3B+ vesicles, and activation of SLAMF1 increased neutrophil autophagy induced by Mtb. Finally, tuberculosis patients? neutrophils displayed reduced levels of SLAMF1 and lower levels of autophagy against Mtb as compared to healthy controls. Altogether, these results indicate that SLAMF1 participates in neutrophil autophagy during active tuberculosis.