Maternal obesogenic diet combined with postnatal exposure to high‐fat diet induces metabolic alterations in offspring

CORREA, FERNANDO; SCHANDER, JULIETA A.; SILBERMAN, DAFNE M.; BARIANI, MARÍA V.; MARVALDI, CAROLINA; CELLA, MAXIMILIANO; FRANCHI, ANA M.; DOMÍNGUEZ RUBIO, ANA P. ; BELTRAME, JIMENA S.; AISEMBERG, JULIETA

JOURNAL OF CELLULAR PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2020 p. 8260 - 8269

0021-9541

Maternal obesity has been shown to impact the offspring health during childhood andadult life. This study aimed to evaluate whether maternal obesity combined withpostnatal exposure to an obesogenic diet could induce metabolic alterations inoffspring. Female CD1 mice were fed a control diet (CD, 11.1% of energy from fat) orwith a high‐fat diet (HFD, 44.3% of energy from fat) for 3 months. After weaning,pups born from control and obese mothers were fed with CD or HFD for 3 months.Both mothers and offspring were weighted weekly and several blood metabolicparameters levels were evaluated. Here, we present evidence that the offspring frommothers exposed to a HFD showed increased acetylation levels of histone 3 on lysine9 (H3K9) in the liver at postnatal Day 1, whereas the levels of acetylation of H4K16,dimethylation of H3K27, and trimethylation of H3K9 showed no change. We alsoobserved a higher perinatal weight and increased blood cholesterol levels whencompared to the offspring on postnatal Day 1 born from CD‐fed mothers. When miceborn from obese mothers were fed with HFD, we observed that they gained moreweight, presented higher blood cholesterol levels, and abdominal adipose tissue thanmice born to the same mothers but fed with CD. Collectively, our results point towardmaternal obesity and HFD consumption as a risk factor for epigenetic changes in theliver of the offspring, higher perinatal weight, increased weight gain, and alteredblood cholesterol levels