High molecular weight chitosan based particles for insulin encapsulation obtained via nanospray technology

MARTÍNEZ, JIMENA H; SAN-JUAN, ERWIN; MAZZOBRE, FLORENCIA; SAN-MARTÍN, EDUARDO; PRUDKIN-SILVA, CECILIA; QUIROZ-REYES, CINTHYA; PÉREZ, OSCAR E.

DRYING TECHNOLOGY

TAYLOR & FRANCIS INC

Año: 2020 p. 1 - 16

0737-3937

The objective of this work was to obtain Chitosan (CS) based particles for Insulin (INS)encapsulation, via nanospray drying of a feeding solution containing equal amounts of bothcomponents (0.1% w/v total solids content). The process was performed at pH 3 which isout of the range for electrostatic interactions to occur. The analysis involved the nanoparticles(NP) characterization in the solution before drying (pH 3) by dynamic light scattering(DLS) and after re-hydration at different pHs (3< pH < 11). The dried product was characterizedby Fourier-transform spectroscopy (FTIR), scanning electron microscopy (SEM) anddifferential scanning calorimetry (DSC). FTIR allowed detecting the chemical groups involvedin INS-CS interactions. The encapsulation efficiency of the glassy NP was 62.3 ± 0.32% asdetermined by HPLC. Upon powder re-hydration, NP of diameter