IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
Autor/es:
FURMENTO, VERONICA; ABAN, CYNTIA; GUBERMAN, ALEJANDRA SONIA; LUZZANI, CARLOS; FURMENTO, VERONICA; ABAN, CYNTIA; GUBERMAN, ALEJANDRA SONIA; LUZZANI, CARLOS; SOLARI, CLAUDIA; LA GRECA, ALEJANDRO; BIANI, MARIA CELESTE; LOMBARDI, ANTONELLA; GARCÍA, MARCELA; MORO, LUCIA; MIRIUKA, SANTIAGO GABRIEL; SEVLEVER, GUSTAVO EMILIO; SOLARI, CLAUDIA; LA GRECA, ALEJANDRO; BIANI, MARIA CELESTE; LOMBARDI, ANTONELLA; GARCÍA, MARCELA; MORO, LUCIA; MIRIUKA, SANTIAGO GABRIEL; SEVLEVER, GUSTAVO EMILIO
Revista:
EXPERIMENTAL AND MOLECULAR MEDICINE EMM
Editorial:
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Referencias:
Año: 2018 vol. 50
ISSN:
1226-3613
Resumen:
Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. Although the therapeutic potential of MSC EVs has already been proven, a thorough characterization of MSC EVs is lacking. In this work, we used a label-free liquid chromatography tandem mass spectrometry proteomic approach to identify the most abundant proteins in EVs that are secreted from MSCs derived from PSCs (PD-MSCs) and from their parental induced PSCs (iPSCs). Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell?substrate adhesion. Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. Correlation and principal component analysis consistently aggregate iPSCs and iPSC EVs but segregate PD-MSC and their EVs. Altogether, these findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties.