Transcriptional regulation of the protein kinase a subunits in Saccharomyces cerevisiae during fermentative growth

GALELLO, FIORELLA; CAÑONERO, LUCIANA; ROSSI, SILVIA; RECA, SOL; MORENO, SILVIA; RECA, SOL; MORENO, SILVIA; PAUTASSO, CONSTANZA; PORTELA, PAULA; PAUTASSO, CONSTANZA; PORTELA, PAULA; GALELLO, FIORELLA; CAÑONERO, LUCIANA; ROSSI, SILVIA

YEAST

JOHN WILEY & SONS LTD

Año: 2017 vol. 34 p. 495 - 508

0749-503X

Yeast cells can adapt their growth in response to the nutritional environment. Glucose isthe favourite carbon source of Saccharomyces cerevisiae, which prefers a fermentativemetabolism despite the presence of oxygen. When glucose is consumed, the cell switchesto the aerobic metabolism of ethanol, during the so-called diauxic shift. The differencebetween fermentative and aerobic growth is in part mediated by a regulatory mechanismcalled glucose repression. During glucose derepression a profound gene transcriptionalreprogramming occurs and genes involved in the utilization of alternative carbonsources are expressed. Protein kinase A (PKA) controls different physiological responsesfollowing the increment of cAMP as a consequence of a particular stimulus. cAMP?PKAis one of the major pathways involved in the transduction of glucose signalling. In thiswork the regulation of the promoters of the PKA subunits during respiratory and fermentativemetabolism are studied. It is demonstrated that all these promoters are upregulatedin the presence of glycerol as carbon source through the Snf1/Cat8 pathway.However, in the presence of glucose as carbon source, the regulation of each PKA promotersubunits is different and only TPK1 is repressed by the complex Hxk2/Mig1 inthe presence of active Snf1.