IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells.
Autor/es:
OSCAR PEREZ; MARÍA E FARÍAS; CECILIA PRUDKIN SILVA; FEDERICO COLUCCIO LESKOW; JIMENA H MARTÍNEZ; FEDERICO COLUCCIO LESKOW; KARINA D MARTÍNEZ; OSCAR PEREZ; MARÍA E FARÍAS; CECILIA PRUDKIN SILVA; FEDERICO COLUCCIO LESKOW; JIMENA H MARTÍNEZ; FEDERICO COLUCCIO LESKOW; KARINA D MARTÍNEZ
Revista:
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 537 p. 425 - 434
ISSN:
0927-7757
Resumen:
The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexesformation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core?shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosityresults kept relation with different stages of nano-complexation process. Biological activity of I-CS complexeswas studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. ICSnano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nanocomplexes.