IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Generation of iPSC line iPSC-FH2. 1 in hypoxic conditions from human foreskin fibroblasts
Autor/es:
CAROLINA BLUGUERMANN; CARLOS LUZZANI; ALEJANDRA GUBERMAN; LEONARDO ROMORINI; GABRIEL NEIMAN; GUSTAVO E. SEVLEVER; LEONARDO ROMORINI; GABRIEL NEIMAN; GUSTAVO E. SEVLEVER; MARIA QUESTA; CLAUDIA SOLARI; MARIA ELIDA SCASSA; SANTIAGO G. MIRIUKA; MARIA QUESTA; CLAUDIA SOLARI; MARIA ELIDA SCASSA; SANTIAGO G. MIRIUKA; CAROLINA BLUGUERMANN; CARLOS LUZZANI; ALEJANDRA GUBERMAN
Revista:
STEM CELL RESEARCH
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 16 p. 300 - 303
ISSN:
1873-5061
Resumen:
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
