IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Intrinsic role of Galectin-1 in the control of the functional properties of immune cells in a prostate cancer context
Autor/es:
GIRIBALDI, LAURA; COMPAGNO, DANIEL; CORAPI, ENRIQUE; JAWORSKI, FELIPE MARTÍN; LADERACH, DIEGO JOSÉ; CARRIZO, GUSTAVO; RABINOVICH, GABRIEL ADRIÁN
Revista:
MEDICINA (BUENOS AIRES)
Editorial:
MEDICINA (BUENOS AIRES)
Referencias:
Año: 2016
ISSN:
0025-7680
Resumen:
The identification of effective new therapies for prostate cancer (PCa) requires a better understanding of the multiple molecular interactions between tumour cells and their associated stroma. In this context, Galectin-1 (Gal-1) plays a major role in determining the properties of the prostatic carcinoma microenvironment. The aim of this study is to elucidate whether Gal-1, in addition to promote tumor neoangiogenesis and immune regulations such as induction of apoptosis on activated T cells, inefficient antigen presentation and expansion of regulatory T cells, plays an additional role as an intrinsic regulator of CD8+ T cell functional properties.To address this concern, we used an in vitro T cell polyclonal activation model combining different cell types (purified by cell sorting) in a prostate tumor microenvironment. Thus, by combination of Gal-1 deficient (Lgals1-/-) and wild type (WT) cells, we were able to assess how the endogenous Gal-1 of each cellular compartment impacts on the CD8+ T cell properties (proliferation and citotoxicity).The absence of Gal-1 in antigen presenting cells (APCs) did not significantly modify the proliferative properties of CD8+T cells. Conversely, the absence of Gal-1 in CD4+ T cells induced a 1.21 fold increase in the proliferation of CD8+T cells. However, the most significant difference in the proliferation was obtained by absence of intrinsic Gal-1 in the CD8+ T cells themselves (2.12). Taking into account Gal-1 controls T cell proliferation, we further evaluated whether Gal-1 is relevant in controlling effector function. Our results demonstrated that upon activation, Lgals1-/- T cells have increased ability to degranulate (evaluated as % (1.87fold) and the content of granules ( 2.48 fold increase)) (p