CONICET | Buscador de Institutos y Recursos Humanos

The insulin is one of the most used peptides worldwide for insulin-dependet patients. Chitosan (CH) is a biodegradable, biocompatible and non-toxic polymer. The objective of this work was to achieve the physicochemical characterization of chemical species mentioned above in order to develop nanoparticulated matrices, constituting nanocarriers for this protein. CHI was characterized in terms of particle size distribution and surface charge obtained on populations with low relative polydispersity. For insulin a monomodal size distribution was corroborated. ζ potencial results equal to 38,4 ±1,2 and y 37,5 ±0,4 mV, for insulin and CHI, respectively at pH 3 Dilute solutions showed a Newtonian behavior for the polysaccharide. These results keep practical implications as it we must have with this information in order to determine and quantify the interaction between these two polyelectrolytes.