IQUIBICEN 23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Agonist mobility on supported lipid bilayers affects Fas mediated death response
Autor/es:
M. FLORENCIA SÁNCHEZ A, VALERIA LEVI B, THOMAS WEIDEMANN C,⇑, DOLORES C. CARRER A,⇑
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2015
ISSN:
0014-5793
Resumen:
Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteinsFas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells,respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, themobility constraints for receptor activation by a membrane embedded agonist is not well understood.We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers,where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Usingdifferent lipid compositions, we show that the apoptotic response correlates with increased lateralmobility of the agonist in the lipid bilayer.
