IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Anti-tissue transglutaminase antibody inhibits apoptotic cell clearanceby macrophages in pregnant NOD mice
Autor/es:
SÓÑORA CECILIA; GUSTAVO MOURGLIA-ETTLIN; GUILLERMINA CALO; VANESA HAUK; ROSANNA RAMHORST; ANA HERNÁNDEZ; CLAUDIA PÉREZ LEIRÓS
Revista:
JOURNAL OF REPRODUCTIVE IMMUNOLOGY.
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 103 p. 59 - 66
ISSN:
0165-0378
Resumen:
Autoimmunity is a feature of celiac disease (CD) with tissue transglutaminase (tTG) as amajor autoantigen. A correlation between gynecological-obstetric disorders in CD patientsand the presence of circulating antibodies anti-tTG that inhibited tTG activity was reported.Serum anti-tTG antibodies were detected in a non-obese diabetic (NOD) mouse model oftype I insulin-dependent diabetes mellitus and Sjögren?s syndrome, two comorbid stateswith CD. Since pregnancy complications have been described in NOD mice, we evaluated theability of anti-tTG antibodies to affect the functions of tTG relevant to the normal course ofan early pregnancy like extracellular matrix assembling and apoptotic cell phagocytosis bymacrophages. Circulating IgG antibodies against tTG were detected in NOD mice with titersthat decreased at early pregnancy; interestingly, the in vitro transamidating activity of tTGwas reduced by NOD serum samples. Particularly, anti-tTG antibody inhibited apoptoticcell phagocytosis by peritoneal macrophages from pregnant NOD mice that express theenzyme on surface. Evidence provided support for a role for anti-tTG antibodies throughreduced transamidating activity and reduced apoptotic cell clearance by the macrophagesof pregnant NOD mice.