IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
Autor/es:
RUIZ, JIMENA ALICIA; GODOY MANUEL S; MEZZINA MARIELA; BIDART GONZALO; MÉNDEZ, BEATRIZ SILVIA; PETTINARI, MARÍA JULIA; NIKEL, PABLO IVÁN
Revista:
Computational and Structural Biotechnology Journal
Editorial:
International Chemical Biology Society
Referencias:
Año: 2012 vol. 3 p. 1 - 10
ISSN:
2001-0370
Resumen:
Bioprocesses conducted under conditions with restricted O2
supply are increasingly exploited for the synthesis of reduced
biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence,
has elaborate sensing and signal transduction mechanisms that respond
to the availability of electron acceptors and alternative carbon sources
in the surrounding environment. In particular, the ArcBA and CreBC
two-component signal transduction systems are largely responsible for
the metabolic regulation of redox control in response to O2
availability, and carbon source utilization, respectively. Significant
advances in the understanding of the biochemical, genetic, and
physiological duties of these regulatory systems have been achieved in
recent years. This situation allowed to rationally-design novel
engineering approaches that ensure optimal carbon and energy flows
within central metabolism, as well as to manipulate redox homeostasis,
in order to optimize the production of industrially-relevant
metabolites. In particular, metabolic flux analysis provided new clues
to understand the metabolic regulation mediated by the ArcBA and CreBC
systems. Genetic manipulation of these regulators proved useful for
designing microbial cells factories tailored for the synthesis of
reduced biochemicals with added value, such as poly(3-hydroxybutyrate),
under conditions with restricted O2 supply. This network-wide
strategy is in contrast with traditional metabolic engineering
approaches, that entail direct modification of the pathways at stake,
and opens new avenues for the targeted modulation of central catabolic
pathways at the transcriptional level.