IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
Differential requirements in endocytic trafficking for dengue virus penetration
Autor/es:
ACOSTA, E.G.; CASTILLA, V.; DAMONTE, E.B.
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2012 vol. 7 p. 1 - 13
ISSN:
1932-6203
Resumen:
The entry of DENV into the host cell appears to be a very complex process which has been started to be studied in detail. In
this report, the route of functional intracellular trafficking after endocytic uptake of dengue virus serotype 1 (DENV-1) strain
HW, DENV-2 strain NGC and DENV-2 strain 16681 into Vero cells was studied by using a susceptibility to ammonium chloride
assay, dominant negative mutants of several members of the family of cellular Rab GTPases that participate in regulation of
transport through endosome vesicles and immunofluorescence colocalization. Together, the results presented demonstrate
that in spite of the different internalization route among viral serotypes in Vero cells and regardless of the viral strain, DENV
particles are first transported to early endosomes in a Rab5-dependent manner. Then a Rab7-dependent pathway guides
DENV-2 16681 to late endosomes, whereas a yet unknown sorting event controls the transport of DENV-2 NGC, and most
probably DENV-1 HW, to the perinuclear recycling compartments where fusion membrane would take place releasing
nucleocapsid into the cytoplasm. Besides the demonstration of a different intracellular trafficking for two DENV-2 strains
that shared the initial clathrin-independent internalization route, these studies proved for the first time the involvement of
the slow recycling pathway for DENV-2 productive infection.