IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
1. Imaging lipid lateral organization in membranes with C-laurdan in a confocal microscop
Autor/es:
MARTÍN DODES, LUIS G FLECHA, VALERIA LEVI.
Revista:
JLR PAPERS IN PRESS
Editorial:
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Referencias:
Lugar: Bethesda, Maryland; Año: 2012 vol. 53 p. 609 - 616
ISSN:
0022-2275
Resumen:
Abstract Lateral organization of biological membranes isfrequently studied using fl uorescence microscopy. One of themost widely used probes for these studies is 2-dimethylamino-6-lauroylnaphthalene (laurdan). The fl uorescence of thisprobe is sensitive to the environment polarity, and thus laurdanreports the local penetration of water when inserted inmembranes. Unfortunately, this probe can only be used undertwo-photon excitation due to its low photostability. Thisis a very important limitation, because there are not too manylaboratories with capability for two-photon microscopy.In this work, we explored the performance of 6-dodecanoyl-2-[ N -methyl- N -(carboxymethyl)amino]naphthalene (C-laurdan),a carboxyl-modifi ed version of laurdan, for imagingbiological membranes using a conventional confocal microscopysetup. We acquired generalized polarization (GP)images of C-laurdan inserted in giant unillamelar vesiclescomposed of binary mixtures of lipids and verifi ed that theprobe allows observing the coexistence of different phases.We also tested the performance of the probe for measurementwith living cells and registered GP images of melanophorecells labeled with C-laurdan in which we could observehighly ordered regions such as fi lopodia. These fi ndingsshow that C-laurdan can be successfully employed for studiesof membrane lateral organization using a conventionalconfocal microscope and can open the possibility of studyinga wide variety of membrane-related processes.— DodesTraian, M. M., L. G. Flecha, and V. Levi. Imaging lipid lateralorganization in membranes with C-laurdan in a confocal