IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
artículos
Título:
The pluripotency transcription factor OCT4 represses heme oxygenase-1 gene expression
Autor/es:
TORO, AYELÉN; SOLARI, CLAUDIA; GUBERMAN, ALEJANDRA; VAZQUEZ ECHEGARAY, CAMILA; COSENTINO, MARIA SOLEDAD; PETRONE, MARÍA VICTORIA; FRANCIA, MARCOS GABRIEL; VAZQUEZ, ELBA
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2021 vol. 595 p. 1949 - 1961
ISSN:
0014-5793
Resumen:
In embryonic stem (ES) cells, oxidative stress control is crucial for genomic stability, self-renewal, and cell differentiation. Heme oxygenase-1 (HO-1) is a key player of the antioxidant system and is also involved in stem cell differentiation and pluripotency acquisition. We found that the HO-1 gene is expressed in ES cells and induced after promoting differentiation. Moreover, downregulation of the pluripotency transcription factor (TF) OCT4 increased HO-1 mRNA levels in ES cells, and analysis of ChIP-seq public data revealed that this TF binds to the HO-1 gene locus in pluripotent cells. Finally, ectopic expression of OCT4 in heterologous systems repressed a reporter carrying the HO-1 gene promoter and the endogenous gene. Hence, this work highlights the connection between pluripotency and redox homeostasis.